Choline Kinase Alpha Rekombinanter Antikörper

Choline Kinase Alpha Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

242095B4

Kat-Nr. : 84881-2-RR

Synonyme

CHKA, EC:2.7.1.32, CKI, CHK, CHETK-alpha

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  WB analysis using 84881-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84881-2-RR (CHKA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84881-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84881-2-RR against Human CHKA were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, COLO 320-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84881-2-RR bindet in WB, ELISA Choline Kinase Alpha und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Choline Kinase Alpha fusion protein Ag4761
• Vollständiger Name: choline kinase alpha
• Berechnetes Molekulargewicht: 439 aa, 52 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC036471
• Gene symbol: CHKA
• Gene ID (NCBI): 1119
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Choline kinase alpha, also named as CHK, CKI and CK, belongs to the choline/ethanolamine kinase family. CHKa may have a regulatory role in phosphatidylcholine synthesis. CHKA catalyze the reaction: ATP + choline = ADP + O-phosphocholine. The human enzyme resembled the rat liver enzyme over the entire sequence. It also resembled the yeast enzyme in the carboxy-terminal region, but not in the amino-terminal region(PMID:1618328).And it expresses in human brain tissue but not in human skeletal muscle(PMID:21665002).It has 2 isoforms produced by alternative splicing.