CHMP2A Polyklonaler Antikörper

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CHMP2A Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10477-1-PBS

Synonyme

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  WB analysis of HepG2 using 10477-1-AP (same clone as 10477-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10477-1-AP (CHMP2A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10477-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10477-1-AP (same clone as 10477-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10477-1-AP (CHMP2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10477-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10477-1-AP (same clone as 10477-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10477-1-AP (CHMP2A Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10477-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 10477-1-AP (same clone as 10477-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10477-1-AP (CHMP2A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10477-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 10477-1-AP (same clone as 10477-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10477-1-AP (CHMP2A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10477-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10477-1-PBS bindet in WB, IHC, Indirect ELISA CHMP2A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHMP2A fusion protein Ag0719
• Vollständiger Name: chromatin modifying protein 2A
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC002502
• Gene symbol: CHMP2A
• Gene ID (NCBI): 27243
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHMP2A, also known as Vps2-1, belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. CHMP2A is a component of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in the degradation of surface receptor proteins and the formation of endocytic multivesicular bodies (MVBs). It has been shown that CHMP2A can interact with and regulate the function of the AAA-ATPase VPS4B (PMID: 15173323). CHMP2A is also reported to be involved in HIV budding (PMID: 21396898; 23051622).

{{ptg:RelatedPrimaryAntibodies}}