CHMP4B Polyklonaler Antikörper

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• KD/KO Validated

CHMP4B Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13683-1-PBS

Synonyme

C20orf178, chromatin modifying protein 4B, Chromatin-modifying protein 4b, hSnf7-2, hVps32-2

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  WB analysis using 13683-1-AP (same clone as 13683-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13683-1-AP (CHMP4B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13683-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 13683-1-AP (same clone as 13683-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13683-1-AP (CHMP4B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13683-1-PBS in a different storage buffer formulation.

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  WB analysis of rat heart using 13683-1-AP (same clone as 13683-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 13683-1-AP (CHMP4B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13683-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 13683-1-AP (same clone as 13683-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13683-1-AP (CHMP4B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13683-1-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 13683-1-AP (same clone as 13683-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using CHMP4B antibody (13683-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13683-1-PBS in a different storage buffer formulation.

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  IF Staining of H9C2 using 13683-1-AP (same clone as 13683-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed H9C2 cells using CHMP4B antibody (13683-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13683-1-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 13683-1-AP (same clone as 13683-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using CHMP4B antibody (13683-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13683-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13683-1-PBS bindet in WB, IF/ICC, Indirect ELISA CHMP4B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHMP4B fusion protein Ag4544
• Vollständiger Name: chromatin modifying protein 4B
• Berechnetes Molekulargewicht: 224 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25-33 kDa
• GenBank-Zugangsnummer: BC033859
• Gene symbol: CHMP4B
• Gene ID (NCBI): 128866
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHMP4B (charged multivesicular body protein 4b) is a human homologue of yeast Snf7p (also named Vps32p) and a member of CHMP family which is implicated in multivesicular body (MVB) sorting. The CHMP proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies. Among seven CHMP family members, only CHMP4 has been found to interact with Alix, a multifunctional adaptor protein in various cellular functions. This antibody is highly specific to the CHMP4B and it doesn't cross-react with other CHMPs as well as other CHMP4s (CHMP4A and CHMP4C). CHMP4B can be detected 25-33 kDa by post-translational modification (PMID:26490837).