CHMP7 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CHMP7 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Rezensionen (1)

Kat-Nr. : 16424-1-AP

Synonyme

Chromatin-modifying protein 7, Chromatin modifying protein 7

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  WB analysis using 16424-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16424-1-AP (CHMP7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16424-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16424-1-AP (CHMP7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spinal cord using 16424-1-AP

  mouse spinal cord tissue were subjected to SDS PAGE followed by western blot with 16424-1-AP (CHMP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spinal cord using 16424-1-AP

  rat spinal cord tissue were subjected to SDS PAGE followed by western blot with 16424-1-AP (CHMP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 16424-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16424-1-AP (CHMP7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 16424-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16424-1-AP (CHMP7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon cancer using 16424-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16424-1-AP (CHMP7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 16424-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CHMP7 antibody (16424-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of A431 using 16424-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CHMP7 antibody (16424-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HEK-293T-Zellen, HeLa-Zellen, Jurkat-Zellen, Maus-Rückenmarkgewebe, Ratten-Rückenmarkgewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 5 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

16424-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA CHMP7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHMP7 fusion protein Ag9625
• Vollständiger Name: CHMP family, member 7
• Berechnetes Molekulargewicht: 453 aa, 51 kDa
• Beobachtetes Molekulargewicht: 51-55 kDa
• GenBank-Zugangsnummer: BC019110
• Gene symbol: CHMP7
• Gene ID (NCBI): 91782
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell; TEX264 drives selective autophagy of DNA lesions to promote DNA repair and cell survival; Authors - Pauline Lascaux
human	WB,IF	Nat Cell Biol; Unrestrained ESCRT-III drives micronuclear catastrophe and chromosome fragmentation.; Authors - Marina Vietri ; KD Validated
human	WB	Dev Cell; CC2D1B Coordinates ESCRT-III Activity during the Mitotic Reformation of the Nuclear Envelope.; Authors - Leandro N Ventimiglia ; KD Validated
human	WB	Dev Cell; The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair.; Authors - Samuel S Wallis ; KD Validated
human	WB	Curr Biol; Membrane Binding by CHMP7 Coordinates ESCRT-III-Dependent Nuclear Envelope Reformation.; Authors - Yolanda Olmos ; KD Validated
human	WB,IP	Elife; CDK1 controls CHMP7-dependent nuclear envelope reformation.; Authors - Alberto T Gatta ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Viviana (Verified Customer) (06-01-2022)

Very good to IP endogenous protein from mouse tissue (2 ug for less than 2mg of total protein tested) and also for western blot.

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse embryonic tissue

CHMP7 Antibody Western Blot,Immunoprecipitation validation (1:1000 dilution) in mouse embryonic tissue (Cat no:16424-1-AP)