CHN1 Polyklonaler Antikörper

CHN1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 12048-1-AP

Synonyme

A chimaerin, Alpha chimerin, ARHGAP2, chimerin (chimaerin) 1, CHN, CHN1, DURS2, N chimaerin, N chimerin, NC, RHOGAP2

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  WB analysis using 12048-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12048-1-AP (CHN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 12048-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 12048-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12048-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12048-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12048-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12048-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12048-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12048-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below

Produktinformation

12048-1-AP bindet in WB, IHC, ELISA CHN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHN1 fusion protein Ag2678
• Vollständiger Name: chimerin (chimaerin) 1
• Berechnetes Molekulargewicht: 459 aa, 53 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC011393
• Gene symbol: CHN1
• Gene ID (NCBI): 1123
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IHC	BMC Cancer; Upregulation of miR-205 induces CHN1 expression, which is associated with the aggressive behaviour of cervical cancer cells and correlated with lymph node metastasis.; Authors - Jianbing Liu
human	WB,IHC	Bioengineered; Bioinformatics-based analysis of the association between the A1-chimaerin (CHN1) gene and gastric cancer; Authors - Jie-Pin Li