CHOP/GADD153 Rekombinanter Antikörper

CHOP/GADD153 Rekombinant Antikörper für WB, Indirect ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

1G11

Kat-Nr. : 81462-1-PBS

Synonyme

CHOP; GADD153, CHOP, DDIT3, GADD153, 1G11

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  WB analysis of HeLa using 81462-1-RR (same clone as 81462-1-PBS)

  Untreated and tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 81462-1-RR (CHOP; GADD153 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81462-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 81462-1-RR (same clone as 81462-1-PBS)

  Untreated and tunicamycin treated Jurkat cells were subjected to SDS PAGE followed by western blot with 81462-1-RR (CHOP; GADD153 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81462-1-PBS in a different storage buffer formulation.

• 

  ChIP experiment of NIH/3T3 using 81462-1-RR (same clone as 81462-1-PBS)

  Chromatin was prepared from NIH/3T3 cells treated with Tunicamycin (2ug/ml, overnigh). Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of CHOP (81462-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 81462-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 81462-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81462-1-RR against Human CHOP/GADD153 were performed. The affinity constant is 104 nM.

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Geprüfte Anwendungen

Produktinformation

81462-1-PBS bindet in WB, Indirect ELISA, ChIP-qPCR CHOP/GADD153 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CHOP/GADD153 fusion protein Ag7354
• Vollständiger Name: DNA-damage-inducible transcript 3
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC003637
• Gene symbol: CHOP
• Gene ID (NCBI): 1649
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).