CHOP; GADD153 Rekombinanter Antikörper

CHOP; GADD153 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1G11

Kat-Nr. : 81462-1-RR

Synonyme

C/EBP homologous protein, C/EBP homologous protein 10, CEBPZ, CHOP, CHOP 10, CHOP10, DDIT 3, DDIT3, GADD153

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 81462-1-RR

  Untreated and tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 81462-1-RR (CHOP; GADD153 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 81462-1-RR

  Untreated and tunicamycin treated Jurkat cells were subjected to SDS PAGE followed by western blot with 81462-1-RR (CHOP; GADD153 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit Tunicamycin behandelte HeLa-Zellen, Tunicamycin treated Jurkat cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

81462-1-RR bindet in WB, ELISA CHOP; GADD153 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CHOP; GADD153 fusion protein Ag7354
• Vollständiger Name: DNA-damage-inducible transcript 3
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC003637
• Gene symbol: DDIT3
• Gene ID (NCBI): 1649
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).