CHRNA3 Polyklonaler Antikörper

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CHRNA3 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10333-1-PBS

Synonyme

Neuronal acetylcholine receptor subunit alpha-3, NACHRA3

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  WB analysis using 10333-1-AP (same clone as 10333-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 10333-1-AP (CHRNA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 10333-1-AP (same clone as 10333-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10333-1-AP (CHRNA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 10333-1-AP (same clone as 10333-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10333-1-AP (CHRNA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10333-1-AP (same clone as 10333-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10333-1-AP (CHRNA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 10333-1-AP (same clone as 10333-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10333-1-AP (CHRNA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10333-1-AP (same clone as 10333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10333-1-AP (CHRNA3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 10333-1-AP (same clone as 10333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10333-1-AP (CHRNA3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 10333-1-AP (same clone as 10333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10333-1-AP (CHRNA3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10333-1-PBS bindet in WB, IHC, Indirect ELISA CHRNA3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHRNA3 fusion protein Ag0272
• Vollständiger Name: cholinergic receptor, nicotinic, alpha 3
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC000513
• Gene symbol: CHRNA3
• Gene ID (NCBI): 1136
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHRNA3, also named as NACHRA3, LNCR2 and PAOD2, is neuronal type nAChR subunits. It is a member of the nicotinic acetylcholine receptor family, which plays an important role in calcium regulation, neuronal development, and cognitive functions. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer.

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