CHRNA7 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CHRNA7 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(23)

Kat-Nr. : 21379-1-AP

Synonyme

Neuronal acetylcholine receptor subunit alpha-7, NACHRA7, CHRNA7 2

---

• 

  WB analysis using 21379-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21379-1-AP (CHRNA7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21379-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21379-1-AP (CHRNA7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 21379-1-AP

  WB result of CHRNA7 antibody (21379-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHRNA7 transfected Jurkat cells.

• 

  WB analysis using 21379-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21379-1-AP (CHRNA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 21379-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21379-1-AP (CHRNA7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 21379-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21379-1-AP (CHRNA7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 21379-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21379-1-AP (CHRNA7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21379-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21379-1-AP (CHRNA7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 21379-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21379-1-AP (CHRNA7 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 21379-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21379-1-AP (CHRNA7 Antibody) at dilution of 1:200 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HL-60-Zellen, MCF-7-Zellen, Maus-Skelettmuskelgewebe, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 19 publications below
• IHC: See 3 publications below
• IF: See 6 publications below
• FC: See 1 publications below

Produktinformation

21379-1-AP bindet in WB, IHC, IF, ELISA CHRNA7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHRNA7 fusion protein Ag15980
• Vollständiger Name: cholinergic receptor, nicotinic, alpha 7
• Berechnetes Molekulargewicht: 502 aa, 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC037571
• Gene symbol: CHRNA7
• Gene ID (NCBI): 1139
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nicotinic acetylcholine receptors (nAChRs) are cholinergic receptors that form ligand-gated ion channels that mediate fast signal transmission at synapses. CHRNA7 (neuronal acetylcholine receptor subunit alpha-7) forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. CHRNA7, so that like CHRNA7, the epithelial CHRFAM7A ORF encodes a protein (48 kDa) that differs from the CHRNA7 (58 kDa) by a distinct amino terminus and molecular weight.(PMID: 25473097)

Publikationen

Species	Application	Title
mouse	WB	J Neuroinflammation; Kynurenic acid blunts A1 astrocyte activation against neurodegeneration in HIV-associated neurocognitive disorders; Authors - Jingxian Lun
mouse	WB,IHC	Biomed Pharmacother; Sinomenine regulates the cholinergic anti-inflammatory pathway to inhibit TLR4/NF-κB pathway and protect the homeostasis in brain and gut in scopolamine-induced Alzheimer's disease mice; Authors - Haojie Ni
human	WB	J Invest Dermatol; Chrna5 is overexpressed in psoriasis patients and promotes psoriasis-like inflammation in mouse models.; Authors - Jing Wang
mouse	WB,IF	Front Pharmacol; Autophagy is Involved in Neuroprotective Effect of Alpha7 Nicotinic Acetylcholine Receptor on Ischemic Stroke.; Authors - Zhe-Qi Xu
rat	WB,IF	Front Pharmacol; Activation of α7nAChR Protects Against Gastric Inflammation and Dysmotility in Parkinson's Disease Rats.; Authors - Li Zhou
mouse	WB	J Alzheimers Dis; Gx-50 Inhibits Neuroinflammation via α7 nAChR Activation of the JAK2/STAT3 and PI3K/AKT Pathways; Authors - Shi Shi