CHRNA7 (48 kDa) Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

CHRNA7 (48 kDa) Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

2A10

Kat-Nr. : 82848-3-RR

Synonyme

CHRNA7, Neuronal acetylcholine receptor subunit alpha-7, NACHRA7, CHRNA7 2, 2A10

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  WB analysis using 82848-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82848-3-RR (CHRNA7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 82848-3-RR

  WB result of CHRNA7 antibody (82848-3-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHRNA7 transfected Jurkat cells.

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  IHC staining of mouse brain using 82848-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82848-3-RR (CHRNA7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 82848-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82848-3-RR (CHRNA7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat dorsal root ganglion using 82848-3-RR

  Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 82848-3-RR (CHRNA7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat dorsal root ganglion using 82848-3-RR

  Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 82848-3-RR (CHRNA7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 82848-3-RR

  Biolayer interferometry (BLl) kinetic assays of 82848-3-RR against Human CHRNA7 (48 kDa) were performed. The affinity constant is 2.04 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, rat dorsal root ganglion tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

82848-3-RR bindet in WB, IHC, ELISA CHRNA7 (48 kDa) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CHRNA7 (48 kDa) fusion protein Ag15980
• Vollständiger Name: cholinergic receptor, nicotinic, alpha 7
• Berechnetes Molekulargewicht: 502 aa, 56 kDa
• Beobachtetes Molekulargewicht: ~50 kDa
• GenBank-Zugangsnummer: BC037571
• Gene symbol: CHRNA7
• Gene ID (NCBI): 1139
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nicotinic acetylcholine receptors (nAChRs) are cholinergic receptors that form ligand-gated ion channels that mediate fast signal transmission at synapses. CHRNA7 (neuronal acetylcholine receptor subunit alpha-7) forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. CHRNA7, so that like CHRNA7, the epithelial CHRFAM7A ORF encodes a protein (48 kDa) that differs from the CHRNA7 (58 kDa) by a distinct amino terminus and molecular weight.(PMID: 25473097)

Publikationen

Species	Application	Title
human	WB	Free Radic Biol Med; Ultrasound-Responsive Taurine Lipid Nanoparticles Attenuate Oxidative Stress and Promote Macrophage Polarization for Diabetic Wound Healing; Authors - Zucheng Luo