CHRNB4 Polyklonaler Antikörper

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• KD/KO Validated

CHRNB4 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 22192-1-AP

Synonyme

CHRNB 4, Neuronal acetylcholine receptor subunit beta-4

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  WB analysis of SH-SY5Y using 22192-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22192-1-AP (CHRNB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22192-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22192-1-AP (CHRNB4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 22192-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22192-1-AP (CHRNB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  FC experiment of SH-SY5Y using 22192-1-AP

  1x10^6 SH-SY5Y cells were surface stained with 0.4 ug CHRNB4 Polyclonal antibody (22192-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, HeLa-Zellen, Maushodengewebe
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

22192-1-AP bindet in WB, ELISA CHRNB4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHRNB4 fusion protein Ag16767
• Vollständiger Name: cholinergic receptor, nicotinic, beta 4
• Berechnetes Molekulargewicht: 498 aa, 56 kDa
• Beobachtetes Molekulargewicht: 48-60 kDa
• GenBank-Zugangsnummer: BC096080
• Gene symbol: CHRNB4
• Gene ID (NCBI): 1143
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human		Mar Drugs; Differential Expression of Nicotine Acetylcholine Receptors Associates with Human Breast Cancer and Mediates Antitumor Activity of αO-Conotoxin GeXIVA.; Authors - Zhihua Sun
human	WB	Eur J Pharmacol; Identification of nicotinic acetylcholine receptor subunits in different lung cancer cell lines and the inhibitory effect of alpha-conotoxin TxID on lung cancer cell growth.; Authors - Jiang Qian
human	WB	Cancers (Basel); An Integrated Genomic Strategy to Identify CHRNB4 as a Diagnostic/Prognostic Biomarker for Targeted Therapy in Head and Neck Cancer.; Authors - Yi-Hsuan Chuang ; KO Validated
human	WB	Mar Drugs; Cervical Cancer Correlates with the Differential Expression of Nicotinic Acetylcholine Receptors and Reveals Therapeutic Targets.; Authors - Yiqiao Liu