CHST13-Specific Polyklonaler Antikörper

CHST13-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20141-1-PBS

Synonyme

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  WB analysis of mouse liver using 20141-1-AP (same clone as 20141-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20141-1-AP (CHST13-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20141-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 20141-1-AP (same clone as 20141-1-PBS)

  IP result of anti-CHST13-Specific (IP:20141-1-AP, 4ug; Detection:20141-1-AP 1:500) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 20141-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 20141-1-AP (same clone as 20141-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 20141-1-AP (CHST13-Specific antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20141-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 20141-1-AP (same clone as 20141-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 20141-1-AP (CHST13-Specific antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20141-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 20141-1-AP (same clone as 20141-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20141-1-AP (CHST13-Specific antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20141-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human hepatocirrhosis using 20141-1-AP (same clone as 20141-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20141-1-AP (CHST13-Specific antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20141-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 20141-1-AP (same clone as 20141-1-PBS)

  Immunofluorescent analysis of Hela cells, using CHST13 antibody 20141-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20141-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20141-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA CHST13-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: carbohydrate (chondroitin 4) sulfotransferase 13
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: NM_152889
• Gene symbol: CHST13
• Gene ID (NCBI): 166012
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHST13, also named as C4ST3, belongs to the sulfotransferase 2 family. CHST13 catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. CHST13 transfers sulfate to the C4 hydroxyl of beta1,4-linked GalNAc that is substituted with a beta-linked glucuronic acid at the C-3 hydroxyl. No activity toward dermatan. The antibody is specific to CHST13.