CIB1 Monoklonaler Antikörper

CIB1 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte, Rind

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D2C12

Kat-Nr. : 67901-1-PBS

Synonyme

DNA PKcs interacting protein, CIBP, CIB, Calmyrin, Calcium and integrin-binding protein 1

---

• 

  WB analysis using 67901-1-Ig (same clone as 67901-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67901-1-Ig (CIB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67901-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67901-1-Ig (same clone as 67901-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67901-1-Ig (CIB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67901-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67901-1-Ig (same clone as 67901-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67901-1-Ig (CIB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67901-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 67901-1-Ig (same clone as 67901-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.4 ug CIB1 Monoclonal antibody (67901-1-Ig, Clone:1D2C12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67901-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

67901-1-PBS bindet in WB, IHC, Indirect ELISA CIB1 und zeigt Reaktivität mit human, Maus, Ratten, Rind

• Getestete Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CIB1 fusion protein Ag31174
• Vollständiger Name: calcium and integrin binding 1 (calmyrin)
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC000846
• Gene symbol: CIB1
• Gene ID (NCBI): 10519
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.