CILP2 Polyklonaler Antikörper

CILP2 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11813-1-PBS

Synonyme

CILP-2, Cartilage intermediate layer protein 2 C2, Cartilage intermediate layer protein 2 C1

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  WB analysis using 11813-1-AP (same clone as 11813-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11813-1-AP (CILP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11813-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 11813-1-AP (same clone as 11813-1-PBS)

  Immunofluorescent analysis of HEK-293 cells using 11813-1-AP (CILP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11813-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 11813-1-AP (same clone as 11813-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CILP2 antibody (11813-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11813-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11813-1-PBS bindet in WB, IF/ICC, Indirect ELISA CILP2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CILP2 fusion protein Ag2366
• Vollständiger Name: cartilage intermediate layer protein 2
• Berechnetes Molekulargewicht: 126 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC034926
• Gene symbol: CILP2
• Gene ID (NCBI): 148113
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CILP2, also named as Cartilage intermediate layer protein 2, is a 1156 amino acid protein, which is expressed in articular chondrocytes but not in knee meniscal cartilage cells. CILP2 localizes to the intermediate to deep zone of articular cartilage. CILP2 may play a role in cartilage scaffolding. Given the full-length molecular mass of CILP2 is 125 kDa, but the most CILP2 protein in articular cartilage is cleaved,mostly likely at the furin cleavage site. CILP2 protein is detected as a major band on SDS-page migrating at about 90 kDa. (PMID: 21880736)