CIN85 Polyklonaler Antikörper

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• KD/KO Validated

CIN85 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12132-1-PBS

Synonyme

SH3KBP1, HSB-1, HSB 1, CD2BP3, CD2-binding protein 3

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  WB analysis using 12132-1-AP (same clone as 12132-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12132-1-AP (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12132-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 12132-1-AP (same clone as 12132-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12132-1-AP (CIN85 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12132-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 12132-1-AP (same clone as 12132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12132-1-AP (CIN85 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12132-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 12132-1-AP (same clone as 12132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12132-1-AP (CIN85 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12132-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 12132-1-AP (same clone as 12132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12132-1-AP (CIN85 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12132-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12132-1-PBS bindet in WB, IHC, Indirect ELISA CIN85 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CIN85 fusion protein Ag2777
• Vollständiger Name: SH3-domain kinase binding protein 1
• Berechnetes Molekulargewicht: 665 aa, 73 kDa
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC015806
• Gene symbol: SH3KBP1/CIN85
• Gene ID (NCBI): 30011
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CIN85 is identified as a Cbl-interacting protein of 85 kDa and belongs to adaptor/scaffold proteins. It composed of three N-terminal SH3 domains followed by a proline-rich region and a C-terminal coiled-coil region. In association with c-Cbl (Casitas B-lineage lymphoma), an E3 ubiquitin ligase. As an adaptor protein, CIN85 is involved in Cbl-dependent EGFR internalization, intracellular receptor trafficking, sorting and degradation. Moreover, through the SH3 domains and the proline-rich region, CIN85 is implicated in many protein-protein interactions and it is found to play important roles in other processes such as apoptosis, rearrangement of actin cytoskeleton, cell adhesion, immunological synapse, cell migration and invasion.