CIP29 Polyklonaler Antikörper

CIP29 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 15798-1-AP

Synonyme

Proliferation-associated cytokine-inducible protein CIP29, Nuclear protein Hcc-1, HSPC316, HCC1, Cytokine-induced protein of 29 kDa

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  WB analysis using 15798-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15798-1-AP (CIP29 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 15798-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15798-1-AP (CIP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat pancreas using 15798-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 15798-1-AP (CIP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 15798-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15798-1-AP (CIP29 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 15798-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15798-1-AP (CIP29 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 15798-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CIP29 antibody (15798-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 15798-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CIP29 antibody (15798-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, MCF-7-Zellen, Raji-Zellen, Ratten-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1500-1:6000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

15798-1-AP bindet in WB, IHC, IF/ICC, ELISA CIP29 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CIP29 fusion protein Ag8507
• Vollständiger Name: cytokine induced protein 29 kDa
• Berechnetes Molekulargewicht: 210 aa, 24 kDa
• Beobachtetes Molekulargewicht: 29-32 kDa
• GenBank-Zugangsnummer: BC007099
• Gene symbol: CIP29
• Gene ID (NCBI): 84324
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Tumour Biol; Depletion of Dicer promotes epithelial ovarian cancer progression by elevating PDIA3 expression.; Authors - Ying Zhu
human	WB,IF	PLoS Pathog; TREX (transcription/export)-NP complex exerts a dual effect on regulating polymerase activity and replication of influenza A virus; Authors - Lingcai Zhao ; KD Validated