CIRBP Polyklonaler Antikörper

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• KD/KO Validated

CIRBP Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, RIP, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

74

Kat-Nr. : 10209-2-AP

Synonyme

A18 hnRNP, A18HNRNP, CIRBP, CIRP

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Galerie der Validierungsdaten

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  WB analysis of PC-12 using 10209-2-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 10209-2-AP (CIRBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 cells using 10209-2-AP

  WB result of CIRBP antibody (10209-2-AP, 1:500) with si-control and si-CIRBP transfected A549 cell.

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  WB analysis of HepG2 using 10209-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10209-2-AP (CIRBP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10209-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10209-2-AP (CIRBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10209-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10209-2-AP (CIRBP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 10209-2-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 10209-2-AP (CIRBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-13 using 10209-2-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 10209-2-AP (CIRBP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10209-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10209-2-AP (CIRBP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10209-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10209-2-AP (CIRBP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 10209-2-AP

  IP Result of anti-CIRBP (IP:10209-2-AP, 3ug; Detection:10209-2-AP 1:500) with HepG2 cells lysate 6000ug.

• 

  IHC staining of human pancreas cancer using 10209-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10209-2-AP (CIRBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 10209-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10209-2-AP (CIRBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 10209-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10209-2-AP (CIRBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10209-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10209-2-AP (CIRBP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10209-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10209-2-AP (CIRBP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 10209-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10209-2-AP (CIRBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10209-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10209-2-AP (CIRBP antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 10209-2-AP

  Immunofluorescent analysis of HepG2 cells using 10209-2-AP (CIRBP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  FC experiment of A549 using 10209-2-AP

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human CIRBP (10209-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, A549-Zellen, HEK-293-Zellen, HepG2-Zellen, Maushirngewebe, Maushodengewebe, PC-13-Zellen, Y79-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Mammakarzinomgewebe, Maus-Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 19 publications below
• WB: See 60 publications below
• IHC: See 12 publications below
• IF: See 11 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• RIP: See 2 publications below

Produktinformation

10209-2-AP bindet in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA CIRBP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Eichh?rnchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CIRBP fusion protein Ag0280
• Vollständiger Name: cold inducible RNA binding protein
• Berechnetes Molekulargewicht: 172 aa, 19 kDa
• Beobachtetes Molekulargewicht: 19 kDa
• GenBank-Zugangsnummer: BC000403
• Gene symbol: CIRBP
• Gene ID (NCBI): 1153
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CIRBP, also named as A18HNRNP and CIRP, is a cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival[PMID: 19158277]. CIRBP is also involved in cap-independent translation upon moderate cold-shock. It acts as a translational activator. CIRBP is a suppressive rather stimulatory effect on proliferation[PMID: 19777567]. CIRBP can translocate from nucleus to cytoplasm under stresses such as UV irradation or heat shock. Suppression of CIRBP increased the apoptotic cell population of neural stem cells at moderate low temperature[PMID: 20735994]. This antibody (Catalog# 10209-2-AP) is a rabbit polyclonal antibody raised against the full-length CIRBP of human origin.

Publikationen

Species	Application	Title
mouse	WB	Nature; RBM3 mediates structural plasticity and protective effects of cooling in neurodegeneration.; Authors - Diego Peretti
human,squirrel	WB	Cell; iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications.; Authors - Jingxing Ou
rat	WB	Nat Med; Cold-inducible RNA-binding protein (CIRP) triggers inflammatory responses in hemorrhagic shock and sepsis.; Authors - Qiang Xiaoling X
human	WB	Mol Cell; Altered m6A Modification of Specific Cellular Transcripts Affects Flaviviridae Infection.; Authors - Nandan S Gokhale
human	WB	Nucleic Acids Res; Alternative splicing induced by bacterial pore-forming toxins sharpens CIRBP-mediated cell response to Listeria infection; Authors - Morgane Corre
human	WB	EBioMedicine; Hypothermic Preconditioning of Human Cortical Neurons Requires Proteostatic Priming.; Authors - Nina Marie Rzechorzek

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