CIRBP Monoklonaler Antikörper

CIRBP Monoklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Maus / IgM

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

6G3H10

Kat-Nr. : 60025-1-Ig

Synonyme

CIRBP

---

Galerie der Validierungsdaten

• 

  WB analysis of HEK-293 using 60025-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60025-1-Ig (CIRBP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 60025-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60025-1-Ig (CIRBP Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 60025-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60025-1-Ig (CIRBP Antibody) at dilution of 1:50.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

60025-1-Ig bindet in WB, IHC, IF, ELISA CIRBP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Maus / IgM
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CIRBP fusion protein Ag0280
• Vollständiger Name: cold inducible RNA binding protein
• Berechnetes Molekulargewicht: 172 aa, 19 kDa
• GenBank-Zugangsnummer: BC000403
• Gene symbol: CIRBP
• Gene ID (NCBI): 1153
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Thiophiler Affinitätschromatograph
• Lagerungspuffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CIRBP, also named as A18HNRNP and CIRP, is a cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. CIRBP is also involved in cap-independent translation upon moderate cold-shock. It acts as a translational activator. CIRBP is a suppressive rather stimulatory effect on proliferation. It acts as a translational repressor. CIRBP is an immortalized gene in murine primary cells. CIRBP promotes assembly of stress granules (SGs), when overexpressed. It is a potential proto-oncogenic proteins upon overexpression.(PMID:19900510) Suppression of CIRBP increased the apoptotic cell population of neural stem cells at moderate low temperature. (PMID:20735994) This antibody can bind the close sequences genes. This antibody is a mouse monoclonal antibody raised against full length CIRBP of human origin.

Publikationen

Species	Application	Title
rat	IF	Front Pharmacol; Hypothermic Shock Applied After Perinatal Asphyxia Prevents Retinal Damage in Rats.; Authors - Manuel Rey-Funes
rat	WB	PLoS One; Cold Shock Proteins Are Expressed in the Retina Following Exposure to Low Temperatures.; Authors - Ignacio M Larrayoz
rat	WB	Bioorg Med Chem; In silico identification and in vivo characterization of small molecule therapeutic hypothermia mimetics.; Authors - Claire Coderch