CIT Polyklonaler Antikörper

CIT Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 20033-1-AP

Synonyme

CIT, Citron Rho interacting kinase, CRIK, KIAA0949, STK21

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Galerie der Validierungsdaten

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  WB analysis of A431 using 20033-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20033-1-AP (CIT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 20033-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20033-1-AP (CIT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20033-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20033-1-AP (CIT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20033-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20033-1-AP (CIT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 20033-1-AP

  Immunofluorescent analysis of HepG2 cells, using CIT antibody 20033-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

20033-1-AP bindet in WB, IF, ELISA CIT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: citron (rho-interacting, serine/threonine kinase 21)
• Berechnetes Molekulargewicht: 231 kDa
• Beobachtetes Molekulargewicht: 230 kDa, 177 kDa
• GenBank-Zugangsnummer: NM_007174
• Gene symbol: CIT
• Gene ID (NCBI): 11113
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CIT, also named KIAA0949, STK21 and CRIK, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. It is required for KIF14 localization to the central spindle and midbody. CIT may play a role in cytokinesis. Putative RHO/RAC effector that binds to the GTP-bound forms of RHO and RAC1. It probably binds p21 with a tighter specificity in vivo. CIT plays an important role in the regulation of cytokinesis and the development of the central nervous system. The antibody recognizes all the isoforms of CIT.

Publikationen

Species	Application	Title
human	WB	Int J Mol Sci; Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.; Authors - Federica Morani
mouse	WB	Mol Cell Biol; E2F-2 Promotes Nuclear Condensation and Enucleation of Terminally Differentiated Erythroblasts.; Authors - Kelsey L Swartz
human	WB	Sci Rep; A three-gene signature reveals changes in the tumor immune microenvironment in the progression from NAFLD to HCC; Authors - Lijuan Liu