CIT Polyklonaler Antikörper

CIT Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20033-1-PBS

Synonyme

---

• 

  WB analysis of A431 using 20033-1-AP (same clone as 20033-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 20033-1-AP (CIT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20033-1-AP (same clone as 20033-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20033-1-AP (CIT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 20033-1-AP (same clone as 20033-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20033-1-AP (CIT antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 20033-1-AP (same clone as 20033-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20033-1-AP (CIT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 20033-1-AP (same clone as 20033-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20033-1-AP (CIT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 20033-1-AP (same clone as 20033-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using CIT antibody 20033-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

20033-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CIT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: citron (rho-interacting, serine/threonine kinase 21)
• Berechnetes Molekulargewicht: 231 kDa
• Beobachtetes Molekulargewicht: 230 kDa, 177 kDa
• GenBank-Zugangsnummer: NM_007174
• Gene symbol: CIT
• Gene ID (NCBI): 11113
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CIT, also named KIAA0949, STK21 and CRIK, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. It is required for KIF14 localization to the central spindle and midbody. CIT may play a role in cytokinesis. Putative RHO/RAC effector that binds to the GTP-bound forms of RHO and RAC1. It probably binds p21 with a tighter specificity in vivo. CIT plays an important role in the regulation of cytokinesis and the development of the central nervous system. The antibody recognizes all the isoforms of CIT.