CITED2 Polyklonaler Antikörper

CITED2 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15021-1-PBS

Synonyme

MSG-related protein 1, MSG related protein 1, MRG-1, MRG1, MRG 1

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  WB analysis of SH-SY5Y using 15021-1-AP (same clone as 15021-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15021-1-AP (CITED2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15021-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 15021-1-AP (same clone as 15021-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15021-1-AP (CITED2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15021-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 15021-1-AP (same clone as 15021-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15021-1-AP (CITED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15021-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 15021-1-AP (same clone as 15021-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15021-1-AP (CITED2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15021-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 15021-1-AP (same clone as 15021-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 15021-1-AP (CITED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15021-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 15021-1-AP (same clone as 15021-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed SH-SY5Y cells using 15021-1-AP (CITED2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15021-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15021-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CITED2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CITED2 fusion protein Ag7078
• Vollständiger Name: Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 36 kDa, 27 kDa
• GenBank-Zugangsnummer: BC004377
• Gene symbol: CITED2
• Gene ID (NCBI): 10370
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CITED2, also named as P35srj, is a cAMP-responsive element-binding protein (CBP)/p300-transactivator, with an ED(Glu/Asp)-rich C-terminal domain that acts as an important modulator in the development of the heart [PMID:10552932]. There are two isoforms of CITED2 protein and the calculated molecular weight of them are 24kDa and 28 kDa. The 28 kDa isoform having modification site may migrate to 35 kDa and 24 kDa isoform having modification site may migrate to 27 kDa (PMID: 23082118). It is proposed to be a negative regulator of HIF-1α through its competitive binding to the CH1 domain of CBP/p300, which mediated signaling and to a decrease in HIF-1-responsive genes such as VEGF [PMID:9887100]. In addition, it is also a co-activator of the transcriptional activity of the TFAP2 isoform, which is an extensively expressed nuclear protein with functions in embryogenesis, inflammation, and stress responses[PMID:22735262].