CKAP2L Rekombinanter Antikörper

CKAP2L Rekombinant Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250860G10

Kat-Nr. : 86247-1-PBS

Synonyme

Cytoskeleton-associated protein 2-like, Radial fiber and mitotic spindle protein, Radmis

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  WB analysis using 86247-1-RR (same clone as 86247-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86247-1-RR (CKAP2L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86247-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 86247-1-RR (same clone as 86247-1-PBS)

  IP result of anti-CKAP2L (IP:86247-1-RR, 4ug; Detection:86247-1-RR 1:1000) with Jurkat cells lysate 1360 ug. This data was developed using the same antibody clone with 86247-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 86247-1-RR (same clone as 86247-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CKAP2L antibody (86247-1-RR, Clone: 250860G10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86247-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

86247-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA CKAP2L und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CKAP2L fusion protein Ag10909
• Vollständiger Name: cytoskeleton associated protein 2-like
• Berechnetes Molekulargewicht: 745 aa, 84 kDa
• Beobachtetes Molekulargewicht: 84 kDa
• GenBank-Zugangsnummer: BC036217
• Gene symbol: CKAP2L
• Gene ID (NCBI): 150468
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CKAP2L is a microtubule-binding protein that is crucial for the formation of the mitotic spindle and the cell cycle progression of neural progenitor cells. Mutations in CKAP2L have been associated with spindle organization defects, including mitotic spindle defects, lagging chromosomes, and chromatin bridges. There is evidence that mutations in this gene are associated with Filippi syndrome, characterized by growth defects, microcephaly, intellectual disability, facial feature defects, and syndactyly(PMID: 25439729).