CKAP2L Rekombinanter Antikörper

CKAP2L Rekombinant Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

250860G10

Kat-Nr. : 86247-1-RR

Synonyme

Cytoskeleton-associated protein 2-like, Radial fiber and mitotic spindle protein, Radmis

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  WB analysis using 86247-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86247-1-RR (CKAP2L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 86247-1-RR

  IP result of anti-CKAP2L (IP:86247-1-RR, 4ug; Detection:86247-1-RR 1:1000) with Jurkat cells lysate 1360 ug.

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  IF Staining of HepG2 using 86247-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CKAP2L antibody (86247-1-RR, Clone: 250860G10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HepG2-Zellen, Jurkat-Zellen, Raji-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86247-1-RR bindet in WB, IF/ICC, IP, ELISA CKAP2L und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CKAP2L fusion protein Ag10909
• Vollständiger Name: cytoskeleton associated protein 2-like
• Berechnetes Molekulargewicht: 745 aa, 84 kDa
• Beobachtetes Molekulargewicht: 84 kDa
• GenBank-Zugangsnummer: BC036217
• Gene symbol: CKAP2L
• Gene ID (NCBI): 150468
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CKAP2L is a microtubule-binding protein that is crucial for the formation of the mitotic spindle and the cell cycle progression of neural progenitor cells. Mutations in CKAP2L have been associated with spindle organization defects, including mitotic spindle defects, lagging chromosomes, and chromatin bridges. There is evidence that mutations in this gene are associated with Filippi syndrome, characterized by growth defects, microcephaly, intellectual disability, facial feature defects, and syndactyly(PMID: 25439729).