CKB/CKM Monoklonaler Antikörper

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• KD/KO Validated

CKB/CKM Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

2B7C3

Kat-Nr. : 66764-1-Ig

Synonyme

CKB, KCRB, 2B7C3, B CK, B-CK

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  WB analysis of HEK-293 using 66764-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66764-1-Ig (CKB/CKM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66764-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66764-1-Ig (CKB/CKM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 66764-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66764-1-Ig (CKB/CKM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66764-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66764-1-Ig (CKB/CKM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:10000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

66764-1-Ig bindet in WB, IHC, ELISA CKB/CKM und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CKB/CKM fusion protein Ag7933
• Vollständiger Name: creatine kinase, brain
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC001190
• Gene symbol: CKB
• Gene ID (NCBI): 1152
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CKBB, also named as B-CK and CKB, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis. CKBB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. This antibody can recognize both CKB and CKM due to the high homology.

Publikationen

Species	Application	Title
human	IHC	Theranostics; Single-cell analyses reveal evolution mimicry during the specification of breast cancer subtype; Authors - Zhi-Jie Gao
human	WB	Pharmacol Res; Ginkgolide B targets and inhibits creatine kinase B to regulate the CCT/TRiC-SK1 axis and exerts pro-angiogenic activity in middle cerebral artery occlusion mice.; Authors - Jiale Zhu ; KD Validated
human	WB	Transl Oncol; RNA-binding protein IGF2BP2 suppresses metastasis of clear cell renal cell carcinoma by enhancing CKB mRNA stability and expression; Authors - Junwu Ren