CKB/CKM Monoklonaler Antikörper

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• KD/KO Validated

CKB/CKM Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2B7C3

Kat-Nr. : 66764-1-PBS

Synonyme

CKB, KCRB, 2B7C3, B CK, B-CK

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• 

  WB analysis of HEK-293 using 66764-1-Ig (same clone as 66764-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66764-1-Ig (CKB/CKM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66764-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66764-1-Ig (same clone as 66764-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66764-1-Ig (CKB/CKM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66764-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66764-1-Ig (same clone as 66764-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66764-1-Ig (CKB/CKM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66764-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66764-1-Ig (same clone as 66764-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66764-1-Ig (CKB/CKM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66764-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66764-1-PBS bindet in WB, IHC, Indirect ELISA CKB/CKM und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CKB/CKM fusion protein Ag7933
• Vollständiger Name: creatine kinase, brain
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC001190
• Gene symbol: CKB
• Gene ID (NCBI): 1152
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CKBB, also named as B-CK and CKB, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis. CKBB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. This antibody can recognize both CKB and CKM due to the high homology.