CKB-Specific Rekombinanter Antikörper

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• KD/KO Validated

CKB-Specific Rekombinant Antikörper für WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240418D2

Kat-Nr. : 83522-5-PBS

Synonyme

CKB, KCRB, 240418D2, B CK, B-CK

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  WB analysis using 83522-5-RR (same clone as 83522-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83522-5-RR (CKB-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83522-5-PBS in a different storage buffer formulation.

• 

  WB analysis of HAP1 using 83522-5-RR (same clone as 83522-5-PBS)

  WB result of sg-CKB-Specific antibody (83522-5-RR; 1:10000; room temperature for 1.5 hours) with wild-type and sg-CKB-Specific transfected HAP1 cells. This data was developed using the same antibody clone with 83522-5-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA sample test of MP00504-4

  The mean CKB concentration was determined to be 138.08 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load, 159.52 ng/mL in HeLa cell extract based on a 1.30 mg/mL extract load, 161.58 ng/mL in mouse brain tissue extract based on a 6.60 mg/mL extract load and 200.17 ng/mL in rat brain tissue extract based on a 8.20 mg/mL extract load.

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  Sandwich ELISA standard curve of MP00504-4

  Sandwich ELISA standard curve of MP00504-4, Human CKB Recombinant Matched Antibody Pair - PBS only. 83522-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7285. 83522-6-PBS was HRP conjugated as the detection antibody. Range: 1.56-50 ng/mL.

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  IHC staining of human colon cancer using 83522-5-RR (same clone as 83522-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83522-5-RR (CKB-Specific antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83522-5-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83522-5-RR (same clone as 83522-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CKB-Specific Recombinant Antibody (83522-5-RR, Clone:240418D2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83522-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83522-5-PBS

  Biolayer interferometry (BLl) kinetic assay of 83522-5-PBS against Human CKB-Specific was performed. The affinity constant is 1.54 nM.

• 

  Affinity and Kinetic Characterization of 83522-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83522-5-RR against Human CKB were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83522-5-PBS bindet in WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA CKB-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CKB-Specific fusion protein Ag7285
• Vollständiger Name: creatine kinase, brain
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC001190
• Gene symbol: CKB
• Gene ID (NCBI): 1152
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CKBB, also named as B-CK and CKB, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis. CKBB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.