CKM-Specific Monoklonaler Antikörper

CKM-Specific Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

2G3F6

Kat-Nr. : 60177-1-Ig

Synonyme

CKM, 2G3F6, Creatine kinase M-type, CPK-M, CKMM

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  WB analysis of human skeletal muscle using 60177-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 60177-1-Ig (CKM-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 60177-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 60177-1-Ig (CKM-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 60177-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60177-1-Ig (CKM-Specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 60177-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 60177-1-Ig (CKM-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 60177-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60177-1-Ig (CKM-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 60177-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 60177-1-Ig (CKM-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 60177-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60177-1-Ig (CKM-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 60177-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60177-1-Ig (CKM-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 60177-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CKM-Specific antibody (60177-1-Ig, Clone: 2G3F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of C2C12 using 60177-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using CKM-Specific antibody (60177-1-Ig, Clone: 2G3F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Human skeletal muscle, humanes Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, Mausherzgewebe, Maushodengewebe, Rattenhirngewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

60177-1-Ig bindet in WB, IHC, IF-P, ELISA CKM-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: creatine kinase, muscle
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa, 90 kDa, 130 kDa
• GenBank-Zugangsnummer: BC007462
• Gene symbol: CKM
• Gene ID (NCBI): 1158
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CKM, also named as CKMM and M-CK, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. CKM reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium. CKM has a calculated molecular mass of 43 kDa, and the 90-kDa and 130-kDa bands could be due to acovalent cross-linking of two and three CKm subunits, respectively (PMID: 20195383).

Publikationen

Species	Application	Title
human	WB	Biomed Pharmacother; Novel small molecule DMAMCL induces differentiation in rhabdomyosarcoma by downregulating of DLL1; Authors - Qi Li