CKMT1A Rekombinanter Antikörper

CKMT1A Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240825A4

Kat-Nr. : 83750-4-RR

Synonyme

CKMT1B, CKMT1, CKMT, CKMiMi, Acidic-type mitochondrial creatine kinase

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  WB analysis using 83750-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83750-4-RR (CKMT1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83750-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83750-4-RR against Human CKMT1A were performed. The affinity constant is 2.86 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HepG2-Zellen, Mausherzgewebe, Rattenherzgewebe, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83750-4-RR bindet in WB, ELISA CKMT1A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CKMT1A fusion protein Ag7583
• Vollständiger Name: creatine kinase, mitochondrial 1A
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC001926
• Gene symbol: CKMT1A
• Gene ID (NCBI): 548596
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CKMT1A(Creatine kinase U-type, mitochondrial) is also named CKMT and belongs to the ATP:guanido phosphotransferase family. CKMT1A is a nearly identical copy of the CKMT1B gene. Both genes encode an identical CKMT1 protein(PMID:17098888). It reversibly catalyzes the transfer of phosphate between ATP and various phosphates. It has 2 isoforms produced by alternative splicing with the molecular weight of 47 kDa and 50 kDa.