CKMT2 Polyklonaler Antikörper

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• KD/KO Validated

CKMT2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Rezensionen (1)

Kat-Nr. : 13207-1-AP

Synonyme

Basic-type mitochondrial creatine kinase, CKMT 2, Creatine kinase S-type, mitochondrial, EC:2.7.3.2, Mib CK

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  WB analysis using 13207-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13207-1-AP (CKMT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13207-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13207-1-AP (CKMT2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 13207-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13207-1-AP (CKMT2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 13207-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13207-1-AP (CKMT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 13207-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13207-1-AP (CKMT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 13207-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13207-1-AP (CKMT2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 13207-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13207-1-AP (CKMT2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, humanes Herzgewebe, humanes Skelettmuskelgewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:8000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

13207-1-AP bindet in WB, IHC, IF, CoIP, ELISA CKMT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CKMT2 fusion protein Ag3971
• Vollständiger Name: creatine kinase, mitochondrial 2 (sarcomeric)
• Berechnetes Molekulargewicht: 419 aa, 48 kDa
• Beobachtetes Molekulargewicht: 41-48 kDa
• GenBank-Zugangsnummer: BC029140
• Gene symbol: CKMT2
• Gene ID (NCBI): 1160
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CKMT2 (Creatine kinase S-type, mitochondrial), also known as s-MtCK, is one of the creatine kinase (CK) isozymes that are tightly coupled to adenosine triphosphate (ATP) output via adenine nucleotide transport proteins or carriers, making it an important player in ATP synthesis and respiratory chain activity (PMID: 38172162). Enhancing Ckmt2 expression or activity can protect against myocardial injury caused by ischemia-reperfusion injury (PMID: 39457679).

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; The phytochemical hyperforin triggers thermogenesis in adipose tissue via a Dlat-AMPK signaling axis to curb obesity.; Authors - Suzhen Chen
chicken	WB	Mol Cell Proteomics; Seminal Plasma Proteome as an Indicator of Sperm Dysfunction and Low Sperm Motility in Chickens; Authors - Yunlei Li
mouse	WB,IHC	Metabolism; Sustained mitochondrial biogenesis is essential to maintain caloric restriction-induced beige adipocytes.; Authors - Raja Gopal Reddy Mooli
human	WB	Front Cell Dev Biol; Hypoxia-immune-related microenvironment prognostic signature for osteosarcoma; Authors - Wenshuo Zhang
chicken	WB	Mol Cell Proteomics; Seminal Plasma Proteome as an Indicator of Sperm Dysfunction and Low Sperm Motility in Chickens.; Authors - Yunlei Li
mouse	WB	J Agric Food Chem; Artepillin C, a key component of Brazilian propolis, induces thermogenesis in inguinal white adipose tissue of mice through a creatine metabolism-related thermogenic pathway.; Authors - Sho Nishikawa

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Neeraj (Verified Customer) (08-19-2025)

This antibody performed very well in validating my Western blot analysis following transfection in cell lines. It demonstrated high specificity, especially when compared to a similar antibody from another supplier, which showed non-specific binding. I also used it for immunofluorescence experiments, and the results were satisfactory, with clear and consistent staining

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:1000 for western blot; 1:200 for Immunostaining
• Cell Tissue Type: Retinal sections, organoids, HEK cell lines