CLASP2 Polyklonaler Antikörper

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• KD/KO Validated

CLASP2 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Publikationen

1

Rezensionen (1)

Kat-Nr. : 12942-1-AP

Synonyme

CLASP2, CLIP associating protein 2, hOrbit2, KIAA0627, Protein Orbit homolog 2

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Galerie der Validierungsdaten

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  WB analysis of human brain using 12942-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12942-1-AP (CLASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12942-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12942-1-AP (CLASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 12942-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12942-1-AP (CLASP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12942-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12942-1-AP (CLASP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12942-1-AP

  Immunofluorescent analysis of HepG2 cells using 12942-1-AP (CLASP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 12942-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12942-1-AP (CLASP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 12942-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12942-1-AP (CLASP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

12942-1-AP bindet in WB, IHC, IF,ELISA CLASP2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CLASP2 fusion protein Ag4012
• Vollständiger Name: cytoplasmic linker associated protein 2
• Berechnetes Molekulargewicht: 1294 aa, 141 kDa
• Beobachtetes Molekulargewicht: 140-160 kDa
• GenBank-Zugangsnummer: BC029035
• Gene symbol: CLASP2
• Gene ID (NCBI): 23122
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CLASP2 (CLIP-associating protein 2) is a brain specific protein belongs to CLASP family. CLASP1 and CLASP2 regulate microtubule plus-end dynamics in brine cortex. CLASP2 is required for the orientation of stabilized microtubules toward the leading edge, It may act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms. Phosphorylation of CLASP2 by GSK3B may negatively regulate binding to microtubule lattices in lamella.

Publikationen

Species	Application	Title
mouse	WB	J Bone Miner Res; Regulation of bone resorption and sealing zone formation in osteoclasts occurs through protein kinase B-mediated microtubule stabilization.; Authors - Matsumoto Takumi T

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sarah (Verified Customer) (07-06-2020)

30ug HEK protein loaded, and CLASP2 levels detected for control cells or cells transfected with CLASP2 siRNA

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK 293T

CLASP2 Antibody Western Blot validation (1:500 dilution) in HEK 293T (Cat no:12942-1-AP)