CLCA1 Polyklonaler Antikörper

CLCA1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25291-1-PBS

Synonyme

Calcium-activated chloride channel regulator 1, Calcium-activated chloride channel protein 1, Calcium-activated chloride channel family member 1, CaCC-1, CACC1

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  WB analysis using 25291-1-AP (same clone as 25291-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25291-1-AP (CLCA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25291-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 25291-1-AP (same clone as 25291-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25291-1-AP (CLCA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25291-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 25291-1-AP (same clone as 25291-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25291-1-AP (CLCA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25291-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

25291-1-PBS bindet in WB, IHC, Indirect ELISA CLCA1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CLCA1 fusion protein Ag18176
• Vollständiger Name: chloride channel accessory 1
• Berechnetes Molekulargewicht: 914 aa, 100 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC156805
• Gene symbol: CLCA1
• Gene ID (NCBI): 1179
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calcium-activated chloride channel regulator 1(CLCA1) is a secreted protein, and it has an N-terminal zinc-dependent metallohydrolase domain with a conserved HExxE catalytic motif similar to that found in matrix metalloproteases (MMPs), and "a Disintegrin and metalloproteases" (ADAMs). The only known substrate for CLCA1 is CLCA1 itself as it undergoes intracellular autocatalytic cleavage resulting in two cleavage products which are both secreted. Since a previous study suggested that a band around 75 kDa, corresponding to the N-terminal autocatalytic cleavage product, was observed in mucus from both human and WT mice. In addition, an additional band at 50 kDa was identified in human and WT mouse mucus, suggesting that CLCA1 can be further processed. However, no band corresponding to the full-length un-cleaved CLCA1 could be detected in the human samples, and only a very faint band was observed in WT mouse mucus, indicating that most of the CLCA1 that is secreted into the mucus is fully cleaved in vivo. (PMID: 29885864)