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CLCA1 Rekombinanter Antikörper

CLCA1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240941G4

Kat-Nr. : 83829-7-PBS

Synonyme

Calcium-activated chloride channel protein 1, Calcium-activated chloride channel family member 1, CaCC-1, CACC1, CACC

Filter:
  • Western Blot (WB) analysis of various lysates using CLCA1 Recombinant antibody (83829-7-RR)

    WB analysis using 83829-7-RR (same clone as 83829-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83829-7-RR (CLCA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83829-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83829-7-RR

    Affinity and Kinetic Characterization of 83829-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83829-7-RR against Human CLCA1 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

83829-7-PBS bindet in WB, ELISA CLCA1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CLCA1 fusion protein Ag18176
Vollständiger Name chloride channel accessory 1
Berechnetes Molekulargewicht 914 aa, 100 kDa
Beobachtetes Molekulargewicht50 kDa
GenBank-ZugangsnummerBC156805
Gene symbol CLCA1
Gene ID (NCBI) 1179
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calcium-activated chloride channel regulator 1(CLCA1) is a secreted protein, and it has an N-terminal zinc-dependent metallohydrolase domain with a conserved HExxE catalytic motif similar to that found in matrix metalloproteases (MMPs), and "a Disintegrin and metalloproteases" (ADAMs). The only known substrate for CLCA1 is CLCA1 itself as it undergoes intracellular autocatalytic cleavage resulting in two cleavage products which are both secreted. Since a previous study suggested that a band around 75 kDa, corresponding to the N-terminal autocatalytic cleavage product, was observed in mucus from both human and WT mice. In addition, an additional band at 50 kDa was identified in human and WT mouse mucus, suggesting that CLCA1 can be further processed. However, no band corresponding to the full-length un-cleaved CLCA1 could be detected in the human samples, and only a very faint band was observed in WT mouse mucus, indicating that most of the CLCA1 that is secreted into the mucus is fully cleaved in vivo. (PMID: 29885864)