CLCA1 Rekombinanter Antikörper

CLCA1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240941G4

Kat-Nr. : 83829-7-RR

Synonyme

Calcium-activated chloride channel protein 1, Calcium-activated chloride channel family member 1, CaCC-1, CACC1, CACC

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  WB analysis using 83829-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83829-7-RR (CLCA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83829-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83829-7-RR against Human CLCA1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83829-7-RR bindet in WB, ELISA CLCA1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CLCA1 fusion protein Ag18176
• Vollständiger Name: chloride channel accessory 1
• Berechnetes Molekulargewicht: 914 aa, 100 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC156805
• Gene symbol: CLCA1
• Gene ID (NCBI): 1179
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calcium-activated chloride channel regulator 1(CLCA1) is a secreted protein, and it has an N-terminal zinc-dependent metallohydrolase domain with a conserved HExxE catalytic motif similar to that found in matrix metalloproteases (MMPs), and "a Disintegrin and metalloproteases" (ADAMs). The only known substrate for CLCA1 is CLCA1 itself as it undergoes intracellular autocatalytic cleavage resulting in two cleavage products which are both secreted. Since a previous study suggested that a band around 75 kDa, corresponding to the N-terminal autocatalytic cleavage product, was observed in mucus from both human and WT mice. In addition, an additional band at 50 kDa was identified in human and WT mouse mucus, suggesting that CLCA1 can be further processed. However, no band corresponding to the full-length un-cleaved CLCA1 could be detected in the human samples, and only a very faint band was observed in WT mouse mucus, indicating that most of the CLCA1 that is secreted into the mucus is fully cleaved in vivo. (PMID: 29885864)