CLCC1 Monoklonaler Antikörper

CLCC1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

3C4B4

Kat-Nr. : 68853-7-Ig

Synonyme

Mid-1-related chloride channel protein 1, MCLC, KIAA0761, Chloride channel CLIC-like protein 1, chloride channel CLIC like 1

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  WB analysis of hTERT-RPE1 using 68853-7-Ig

  hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 68853-7-Ig (CLCC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 68853-7-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 68853-7-Ig (CLCC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68853-7-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68853-7-Ig (CLCC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68853-7-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68853-7-Ig (CLCC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HeLa-Zellen, HepG2-Zellen, hTERT-RPE1-Zellen, K-562-Zellen, MDA-MB-468-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68853-7-Ig bindet in WB, ELISA CLCC1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CLCC1 fusion protein Ag24682
• Vollständiger Name: chloride channel CLIC-like 1
• Berechnetes Molekulargewicht: 551 aa, 62 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: BC002939
• Gene symbol: CLCC1
• Gene ID (NCBI): 23155
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CLCC1, also known as MCLC, is a transmembrane protein that localizes to intracellular compartments, including endoplasmic reticulum (ER), and it functions as a chloride channel (PMID: 11279057). It has been shown that loss of CLCC1 results in ER Stress, misfolded protein accumulation, and neurodegeneration (PMID: 25698737). CLCC1 forms homomultimer in the ER membrane and is a pore-forming component of an anion channel. Studies found that disruption of ER ion homeostasis maintained by CLCC1 is one of the causes of neurodegenerative diseases, such as ALS (amyotrophic lateral sclerosis) (PMID: 37142673).