Claudin 7 Polyklonaler Antikörper

Claudin 7 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 29795-1-AP

Synonyme

CEPTRL2, Claudin 7, CLDN 7, CLDN7, CPETRL2, Hs.84359

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  IHC staining of human oesophagus cancer using 29795-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 29795-1-AP (Claudin 7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 29795-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 29795-1-AP (Claudin 7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 29795-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29795-1-AP (Claudin 7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 29795-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29795-1-AP (Claudin 7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 29795-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 29795-1-AP (Claudin 7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 29795-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 29795-1-AP (Claudin 7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Ösophaguskarzinomgewebe, Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 2 publications below

Produktinformation

29795-1-AP bindet in WB, IHC, IF, ELISA Claudin 7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Claudin 7 fusion protein Ag31706
• Vollständiger Name: claudin 7
• Berechnetes Molekulargewicht: 22.4 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC001055
• Gene symbol: Claudin 7
• Gene ID (NCBI): 1366
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 27 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.

Publikationen

Species	Application	Title
mouse	IF	J Agric Food Chem; Lacticaseibacillus rhamnosus KY16 Improves Depression by Promoting Intestinal Secretion of 5-HTP and Altering the Gut Microbiota; Authors - Shicai Xie
mouse	WB,IF	PLoS One; Curcumin ameliorates aging-induced blood-testis barrier disruption by regulating AMPK/mTOR mediated autophagy; Authors - Chen Xue