CLEC12A Rekombinanter Antikörper

CLEC12A Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

CloneNo.

240791B7

Kat-Nr. : FITC-98083

Synonyme

240791B7, C type lectin like molecule 1, CD371, CLL 1, CLL1

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  FC experiment of human peripheral blood leukocytes using FITC-98083

  1x10^6 human peripheral blood leukocytes were surface stained with CoraLite® Plus 647 Anti-Human CD16, and 5 ul FITC Plus Anti-Human CLEC12A Rabbit RecAb (FITC-98083, Clone: 240791B7) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using FITC-98083

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul FITC Plus Anti-Human CLEC12A Rabbit RecAb (FITC-98083, Clone: 240791B7) (red) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: human peripheral blood leukocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-98083 bindet in FC CLEC12A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CLEC12A fusion protein Eg0024
• Vollständiger Name: C-type lectin domain family 12, member A
• Berechnetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC126291
• Gene symbol: CLEC12A
• Gene ID (NCBI): 160364
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CLEC12A, also known as CD371, MICL, DCAL-2 or CLL-1, is a type II transmembrane glycoprotein and a member of the C-type lectin superfamily (PMID: 30401586). This protein, which contains a single C-type lectin-like domain and a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM), is related to LOX-1 and Dectin-1 and is variably spliced and highly N-glycosylated (PMID: 14739280). Its ITIM sequence recruits the tyrosine phosphatases SHP-1 and SHP-2 to negatively regulate Syk signaling. In humans, CLEC12A is mainly expressed on myeloid cells, being found on monocytes, granulocytes, macrophages and DC subsets, and at lower levels on CD4+ T cells (PMID: 32477350). The observed molecular weight of CLEC12A is between 40 and 75 kDa, which is much higher than the predicted molecular weight due to glycosylation (PMID: 14739280).