CLEC14A Rekombinanter Antikörper

CLEC14A Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241206D3

Kat-Nr. : 84327-1-RR

Synonyme

241206D3, C14orf27, CEG1, C-type lectin domain family 14 member A, EGFR-5

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  WB analysis using 84327-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84327-1-RR (CLEC14A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 84327-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84327-1-RR (CLEC14A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 84327-1-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84327-1-RR (CLEC14A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84327-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84327-1-RR against Human CLEC14A were performed. The affinity constant is 8.76 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HUVEC-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84327-1-RR bindet in WB, IHC, ELISA CLEC14A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CLEC14A fusion protein Ag21081
• Vollständiger Name: C-type lectin domain family 14, member A
• Berechnetes Molekulargewicht: 490 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa, 100 kDa
• GenBank-Zugangsnummer: BC031567
• Gene symbol: CLEC14A
• Gene ID (NCBI): 161198
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CLEC14A is a single-pass type I transmembrane protein whose extracellular domain consists of a C-type lectin-like domain (CTLD), a series of EGF-like domains, and a sushi-like domain (PMID: 23644659). CLEC14A is a member of the C-type lectin domain superfamily that also includes endosialin, thrombomodulin and CD93 (PMID: 16336259). It has been reported that CLEC14A is specifically expressed in endothelial cells and mediates cell to cell adhesion (PMID: 21095181). CLEC14A may be involved in tumor angiogenesis (PMID: 21706054; 23644659).