CLEC9A/CD370 Rekombinanter Antikörper

CLEC9A/CD370 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242895F4

Kat-Nr. : 98395-1-PBS

Synonyme

CD370, Clec9a, C-type lectin domain family 9 member A, Dendritic cell natural killer lectin group receptor 1, Dngr-1

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  FC experiment of BALB/c mouse splenocytes using 98395-1-RR (same clone as 98395-1-PBS)

  1x10^6 BALB/c mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CLEC9A Rabbit RecAb (98395-1-RR, Clone:242895F4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9). Cells were then stained with CoraLite® Plus 405 Anti-Mouse CD11c (N418) Mouse IgG2a Recombinant Antibody (CL405-65602, Clone: N418) and APC Anti-Mouse CD8a (5H10-1) (APC-65205, Clone: 5H10-1). CD8+ were gated. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98395-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

98395-1-PBS bindet in FC CLEC9A/CD370 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CLEC9A/CD370 fusion protein Eg2730
• Vollständiger Name: C-type lectin domain family 9, member a
• Berechnetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: NM_001205363.1
• Gene symbol: Clec9a
• Gene ID (NCBI): 232414
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CLEC9A (C-type lectin domain family 9 member A), also known as DNGR1, CD370, is a type II membrane protein that contains C-type lectin domain. The C-type lectin domain family members share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signaling, glycoprotein turnover, and roles in inflammation and immune response. Mouse Clec9A is primarily restricted to dendritic cells (DCs) and selectively expressed in mouse CD8+ conventional DCs (cDCs) and plasmacytoid DCs (pDCs), whereas human CLEC9A is also expressed on human DC subtypes. CLEC9A functions as an activating receptor that recruits Syk kinase and can induce the production of proinflammatory cytokines. (PMID: 25553393; PMID: 15476922; PMID: 18669894; PMID: 18408006)