CLEC9A/CD370 Rekombinanter Antikörper

CLEC9A/CD370 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242895F4

Kat-Nr. : 98395-1-RR

Synonyme

CD370, Clec9a, C-type lectin domain family 9 member A, Dendritic cell natural killer lectin group receptor 1, Dngr-1

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  FC experiment of BALB/c mouse splenocytes using 98395-1-RR

  1x10^6 BALB/c mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CLEC9A Rabbit RecAb (98395-1-RR, Clone:242895F4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9). Cells were then stained with CoraLite® Plus 405 Anti-Mouse CD11c (N418) Mouse IgG2a Recombinant Antibody (CL405-65602, Clone: N418) and APC Anti-Mouse CD8a (5H10-1) (APC-65205, Clone: 5H10-1). CD8+ were gated. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98395-1-RR bindet in FC CLEC9A/CD370 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CLEC9A/CD370 fusion protein Eg2730
• Vollständiger Name: C-type lectin domain family 9, member a
• Berechnetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: NM_001205363.1
• Gene symbol: Clec9a
• Gene ID (NCBI): 232414
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CLEC9A (C-type lectin domain family 9 member A), also known as DNGR1, CD370, is a type II membrane protein that contains C-type lectin domain. The C-type lectin domain family members share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signaling, glycoprotein turnover, and roles in inflammation and immune response. Mouse Clec9A is primarily restricted to dendritic cells (DCs) and selectively expressed in mouse CD8+ conventional DCs (cDCs) and plasmacytoid DCs (pDCs), whereas human CLEC9A is also expressed on human DC subtypes. CLEC9A functions as an activating receptor that recruits Syk kinase and can induce the production of proinflammatory cytokines. (PMID: 25553393; PMID: 15476922; PMID: 18669894; PMID: 18408006)