Calmegin Polyklonaler Antikörper

Calmegin Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 12629-1-AP

Synonyme

calmegin, CLGN

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 12629-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12629-1-AP (Calmegin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12629-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12629-1-AP (Calmegin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12629-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12629-1-AP (Calmegin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12629-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12629-1-AP (Calmegin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12629-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12629-1-AP (Calmegin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 12629-1-AP

  IP Result of anti-Calmegin (IP:12629-1-AP, 4ug; Detection:12629-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

• 

  IHC staining of human testis using 12629-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12629-1-AP (Calmegin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 12629-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12629-1-AP (Calmegin antibody at dilution of 1:50 (under 40x lens).

• 

  FC experiment of Jurkat using 12629-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.20ug Anti-Human Calmegin (12629-1-AP) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Fixed with90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, humanes Hirngewebe, humanes Lungengewebe, Maushirngewebe, Mauslungengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

12629-1-AP bindet in WB, IP, IHC, IF, FC, ELISA Calmegin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Calmegin fusion protein Ag3322
• Vollständiger Name: calmegin
• Berechnetes Molekulargewicht: 610 aa, 70 kDa
• Beobachtetes Molekulargewicht: 93 kDa
• GenBank-Zugangsnummer: BC028357
• Gene symbol: CLGN
• Gene ID (NCBI): 1047
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calmegin, belonging to the calreticulin family, is a testis-specific molecular chaperon of the endoplasmic reticulum playing a key role in spermatogenesis. Western blot analysis of mouse testis showed a single band with an apparent molecular mass of 93 kDa.

Publikationen

Species	Application	Title
mouse	WB	Mol Cell Proteomics; Integrative proteomic and transcriptomic analyses reveal multiple post-transcriptional regulatory mechanisms of mouse spermatogenesis.; Authors - Haiyun Gan
mouse	IF	Sci Rep; Transcription Factor RFX2 Is a Key Regulator of Mouse Spermiogenesis.; Authors - Yujian Wu