CLIC1 Rekombinanter Antikörper

CLIC1 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250143E11

Kat-Nr. : 85831-4-PBS

Synonyme

G6, NCC27

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  WB analysis using 85831-4-RR (same clone as 85831-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85831-4-RR (CLIC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85831-4-PBS in a different storage buffer formulation.

• 

  WB analysis using 85831-4-RR (same clone as 85831-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85831-4-RR (CLIC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85831-4-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 85831-4-RR (same clone as 85831-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 85831-4-RR (CLIC1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85831-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 85831-4-RR (same clone as 85831-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85831-4-RR (CLIC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85831-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 85831-4-RR (same clone as 85831-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CLIC1 antibody (85831-4-RR, Clone: 250143E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85831-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85831-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85831-4-RR against Human CLIC1 were performed. The affinity constant is 22.0 nM.

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Geprüfte Anwendungen

Produktinformation

85831-4-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CLIC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CLIC1 fusion protein Ag6049
• Vollständiger Name: chloride intracellular channel 1
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 27-32 kDa
• GenBank-Zugangsnummer: BC064527
• Gene symbol: CLIC1
• Gene ID (NCBI): 1192
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chloride intracellular channel protein 1 (CLIC1) is a member of the chloride intracellular channel protein family. It plays a crucial role in various cellular processes, including the regulation of chloride ion transport, cell viability, and mitochondrial function. CLIC1 is involved in the formation of membrane-inserted channels that facilitate chloride ion movement, which is essential for maintaining cellular homeostasis and function (PMID: 32116799). Additionally, CLIC1 has been implicated in cancer progression, where it influences cell proliferation, migration, and invasion (PMID: 38027011).