CLIC3 Polyklonaler Antikörper

CLIC3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 15971-1-AP

Synonyme

CLIC3

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  WB analysis using 15971-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15971-1-AP (CLIC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 15971-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15971-1-AP (CLIC3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 15971-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15971-1-AP (CLIC3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 15971-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15971-1-AP (CLIC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 15971-1-AP

  Immunofluorescent analysis of MCF-7 cells, using CLIC3 antibody 15971-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of MCF-7 using 15971-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CLIC3 antibody (15971-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: JAR-Zellen, humanes Plazenta-Gewebe, Mausnierengewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

15971-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA CLIC3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CLIC3 fusion protein Ag8757
• Vollständiger Name: chloride intracellular channel 3
• Berechnetes Molekulargewicht: 236 aa, 26 kDa
• Beobachtetes Molekulargewicht: 27-30 kDa
• GenBank-Zugangsnummer: BC007012
• Gene symbol: CLIC3
• Gene ID (NCBI): 9022
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume (PMID: 9880541). CLIC3 (chloride intracellular channel protein 3) belongs to the chloride channel CLIC family which has seven members based on sequence homology to the p64 chloride channels of bovine kidney microsomes. CLIC proteins lack the membrane-spanning domains characteristic of channel proteins and are largely intracellular. CLIC3 is predominantly localized in the nucleus and has a high expression level in placenta (PMID: 9880541; 17027078). It associates with the C-terminal of ERK7 and may participate in cellular growth control.

Publikationen

Species	Application	Title
rat	IHC	PLoS One; Mechanisms that determine the internal environment of the developing brain: a transcriptomic, functional and ultrastructural approach.; Authors - Liddelow Shane A SA
human	WB	J Cancer; Analysis of Differentially Expressed Genes in a Chinese Cohort of Esophageal Squamous Cell Carcinoma.; Authors - Gang Liu
human,rat	WB	Front Cell Neurosci; Identification of immune-related biomarkers in peripheral blood of schizophrenia using bioinformatic methods and machine learning algorithms; Authors - Xiaoli Zhu
human	WB,IF,CoIP	Cell Death Dis; CLIC3 interacts with NAT10 to inhibit N4-acetylcytidine modification of p21 mRNA and promote bladder cancer progression; Authors - Yujun Shuai ; KD Validated
human	WB	Invest Ophthalmol Vis Sci; Expression Distribution of Keratins in Normal and Pathological Corneas and the Regulatory Role of Krt17 on Limbal Stem Cells; Authors - Min Wang
mouse	WB,IF	Biophys J; Biophysical characterization of anion channels in Mitochondrion-Endoplasmic-Reticulum Contact sites (MERCs); Authors - Shridhar Kiran Sanghvi