CLIC3 Rekombinanter Antikörper

CLIC3 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242326H7

Kat-Nr. : 84759-4-PBS

Synonyme

242326H7

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  WB analysis of JAR using 84759-4-RR (same clone as 84759-4-PBS)

  JAR cells were subjected to SDS PAGE followed by western blot with 84759-4-RR (CLIC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84759-4-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 84759-4-RR (same clone as 84759-4-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 84759-4-RR (CLIC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84759-4-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84759-4-RR (same clone as 84759-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84759-4-RR (CLIC3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84759-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 84759-4-RR (same clone as 84759-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84759-4-RR (CLIC3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84759-4-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 84759-4-RR (same clone as 84759-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CLIC3 antibody (84759-4-RR, Clone: 242326H7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84759-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84759-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84759-4-RR against Human CLIC3 were performed. The affinity constant is 0.634 nM.

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Geprüfte Anwendungen

Produktinformation

84759-4-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CLIC3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CLIC3 fusion protein Ag8757
• Vollständiger Name: chloride intracellular channel 3
• Berechnetes Molekulargewicht: 236 aa, 26 kDa
• Beobachtetes Molekulargewicht: 27-32 kDa
• GenBank-Zugangsnummer: BC007012
• Gene symbol: CLIC3
• Gene ID (NCBI): 9022
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume (PMID: 9880541). CLIC3 (chloride intracellular channel protein 3) belongs to the chloride channel CLIC family which has seven members based on sequence homology to the p64 chloride channels of bovine kidney microsomes. CLIC proteins lack the membrane-spanning domains characteristic of channel proteins and are largely intracellular. CLIC3 is predominantly localized in the nucleus and has a high expression level in placenta (PMID: 9880541; 17027078). It associates with the C-terminal of ERK7 and may participate in cellular growth control.