CLK1 Polyklonaler Antikörper

CLK1 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 20439-1-AP

Synonyme

CDC-like kinase 1, CLK, EC:2.7.12.1, STY

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  WB analysis using 20439-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20439-1-AP (CLK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20439-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20439-1-AP (CLK1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of HeLa using 20439-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20439-1-AP (CLK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 20439-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20439-1-AP (CLK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 20439-1-AP

  IP result of anti-CLK1 (IP:20439-1-AP, 4ug; Detection:20439-1-AP 1:300) with COLO 320 cells lysate 2000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: COLO 320-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

20439-1-AP bindet in WB, IP, ELISA CLK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CLK1 fusion protein Ag14214
• Vollständiger Name: CDC-like kinase 1
• Berechnetes Molekulargewicht: 484 aa, 57 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: BC031549
• Gene symbol: CLK1
• Gene ID (NCBI): 1195
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CLK1 (CDC-like kinase 1) is the dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. It phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. It can phosphorylate: SRSF1, SRSF3 and PTPN1 (PMID: 10480872; 19168442). It also regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells (PMID: 19168442). Elevated CLK1 activity is frequently detected in cancers, where it alters splice isoform balance to promote proliferation, invasion and chemoresistance, making it an emerging therapeutic target.

Publikationen

Species	Application	Title
human	WB	Front Immunol; Machine learning-based selection of immune cell markers in osteosarcoma: prognostic determination and validation of CLK1 in disease progression; Authors - Nan Zhang