CLK1 Polyklonaler Antikörper

CLK1 Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20439-1-PBS

Synonyme

CDC-like kinase 1, CLK, EC:2.7.12.1, STY

---

• 

  WB analysis using 20439-1-AP (same clone as 20439-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20439-1-AP (CLK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20439-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 20439-1-AP (same clone as 20439-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20439-1-AP (CLK1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 20439-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 20439-1-AP (same clone as 20439-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20439-1-AP (CLK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20439-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 20439-1-AP (same clone as 20439-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20439-1-AP (CLK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20439-1-PBS in a different storage buffer formulation.

• 

  IP experiment of COLO 320 using 20439-1-AP (same clone as 20439-1-PBS)

  IP result of anti-CLK1 (IP:20439-1-AP, 4ug; Detection:20439-1-AP 1:300) with COLO 320 cells lysate 2000ug. This data was developed using the same antibody clone with 20439-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

20439-1-PBS bindet in WB, IP, Indirect ELISA CLK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CLK1 fusion protein Ag14214
• Vollständiger Name: CDC-like kinase 1
• Berechnetes Molekulargewicht: 484 aa, 57 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: BC031549
• Gene symbol: CLK1
• Gene ID (NCBI): 1195
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CLK1 (CDC-like kinase 1) is the dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. It phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. It can phosphorylate: SRSF1, SRSF3 and PTPN1 (PMID: 10480872; 19168442). It also regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells (PMID: 19168442). Elevated CLK1 activity is frequently detected in cancers, where it alters splice isoform balance to promote proliferation, invasion and chemoresistance, making it an emerging therapeutic target.