CLOCK Polyklonaler Antikörper

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• KD/KO Validated

CLOCK Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18094-1-PBS

Synonyme

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  WB analysis of HEK-293 using 18094-1-AP (same clone as 18094-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18094-1-AP (CLOCK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18094-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 18094-1-AP (same clone as 18094-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18094-1-AP (CLOCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18094-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 18094-1-AP (same clone as 18094-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 18094-1-AP (CLOCK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18094-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 18094-1-AP (same clone as 18094-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 18094-1-AP (CLOCK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18094-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 18094-1-AP (same clone as 18094-1-PBS)

  IP result of anti-CLOCK (IP:18094-1-AP, 4ug; Detection:18094-1-AP 1:300) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 18094-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 18094-1-AP (same clone as 18094-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18094-1-AP (CLOCK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18094-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 18094-1-AP (same clone as 18094-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18094-1-AP (CLOCK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18094-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 18094-1-AP (same clone as 18094-1-PBS)

  Immunofluorescent analysis of HeLa cells using 18094-1-AP (CLOCK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18094-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18094-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CLOCK und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CLOCK fusion protein Ag12826
• Vollständiger Name: clock homolog (mouse)
• Berechnetes Molekulargewicht: 846 aa, 95 kDa
• Beobachtetes Molekulargewicht: 95-110 kDa
• GenBank-Zugangsnummer: BC041878
• Gene symbol: CLOCK
• Gene ID (NCBI): 9575
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Circadian locomoter output cycles protein kaput (CLOCK), also named as BHLHE8 or KIAA0334, is a 846 amino acid protein, which contains one bHLH domain, one PAC domain, and two PAS domains. CLOCK localizes in the nucleus and cytoplasm. CLOCK) is expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. ARNTL/2-CLOCK heterodimers activate E-box element (5'-CACGTG-3') transcription of a number of proteins of the circadian clock, such as PER1 and PER2. The calculated molecular weight of CLOCK is 95kDa, we detected a 95-110 kDa protein by western blot. Our result is similar to the result of reseach paper (PMID: 30683868).