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CLOCK Polyklonaler Antikörper

CLOCK Polyklonal Antikörper für IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30680-1-PBS

Synonyme

bHLHe8, Circadian locomoter output cycles protein kaput, Class E basic helix-loop-helix protein 8, EC:2.3.1.48, KAT13D

Filter:
  • Immunohistochemistry (IHC) staining of mouse liver tissue using CLOCK Polyclonal antibody (30680-1-AP)

    IHC staining of mouse liver using 30680-1-AP (same clone as 30680-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30680-1-AP (CLOCK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30680-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CLOCK Polyclonal antibody (30680-1-AP)

    IHC staining of mouse liver using 30680-1-AP (same clone as 30680-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30680-1-AP (CLOCK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30680-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CLOCK Polyclonal antibody (30680-1-AP)

    IF Staining of HeLa using 30680-1-AP (same clone as 30680-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CLOCK antibody (30680-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 30680-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

30680-1-PBS bindet in IHC, IF/ICC, Indirect ELISA CLOCK und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CLOCK fusion protein Ag33370
Vollständiger Name clock homolog (mouse)
Berechnetes Molekulargewicht 846 aa, 95 kDa
GenBank-ZugangsnummerBC041878
Gene symbol CLOCK
Gene ID (NCBI) 9575
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Circadian locomoter output cycles protein kaput (CLOCK), also named as BHLHE8 or KIAA0334, is a 846 amino acid protein, which contains one bHLH domain, one PAC domain, and two PAS domains. CLOCK localizes in the nucleus and cytoplasm. CLOCK) is expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. ARNTL/2-CLOCK heterodimers activate E-box element (5'-CACGTG-3') transcription of a number of proteins of the circadian clock, such as PER1 and PER2. (PMID: 30683868).