CLOCK Rekombinanter Antikörper

CLOCK Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1I23

Kat-Nr. : 82829-1-PBS

Synonyme

1I23, bHLHe8, Circadian locomoter output cycles protein kaput, Class E basic helix-loop-helix protein 8, EC:2.3.1.48

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  WB analysis using 82829-1-RR (same clone as 82829-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82829-1-RR (CLOCK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82829-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 82829-1-RR (same clone as 82829-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82829-1-RR (CLOCK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82829-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82829-1-RR (same clone as 82829-1-PBS)

  WB result of CLOCK antibody (82829-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLOCK transfected HeLa cells. This data was developed using the same antibody clone with 82829-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 82829-1-RR (same clone as 82829-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82829-1-RR (CLOCK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82829-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 82829-1-RR (same clone as 82829-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82829-1-RR (CLOCK antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82829-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82829-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82829-1-RR against Human CLOCK were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

82829-1-PBS bindet in WB, IHC, Indirect ELISA CLOCK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CLOCK fusion protein Ag30011
• Vollständiger Name: clock homolog (mouse)
• Berechnetes Molekulargewicht: 846 aa, 95 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC041878
• Gene symbol: CLOCK
• Gene ID (NCBI): 9575
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Circadian locomoter output cycles protein kaput (CLOCK), also named as BHLHE8 or KIAA0334, is a 846 amino acid protein, which contains one bHLH domain, one PAC domain, and two PAS domains. CLOCK localizes in the nucleus and cytoplasm. CLOCK) is expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. ARNTL/2-CLOCK heterodimers activate E-box element (5'-CACGTG-3') transcription of a number of proteins of the circadian clock, such as PER1 and PER2. The calculated molecular weight of CLOCK is 95kDa, we detected a 95-110 kDa protein by western blot. Our result is similar to the result of reseach paper (PMID: 30683868).