CLPS Rekombinanter Antikörper

CLPS Rekombinant Antikörper für WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241565H3

Kat-Nr. : 84224-2-PBS

Synonyme

Colipase, 241565H3

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  WB analysis of rat pancreas using 84224-2-RR (same clone as 84224-2-PBS)

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 84224-2-RR (CLPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84224-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 84224-2-RR (same clone as 84224-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84224-2-RR (CLPS antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84224-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 84224-2-RR (same clone as 84224-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84224-2-RR (CLPS antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84224-2-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pancreas using 84224-2-RR (same clone as 84224-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CLPS antibody (84224-2-RR, Clone: 241565H3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84224-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01160-1

  Cytometric bead array standard curve of MP01160-1, CLPS Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84224-1-PBS. Detection antibody: 84224-2-PBS. Standard: Ag2321. Range: 0.781-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 84224-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84224-2-RR against Human CLPS were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84224-2-PBS bindet in WB, IHC, IF-P, Cytometric bead array, Indirect ELISA CLPS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CLPS fusion protein Ag2321
• Vollständiger Name: colipase, pancreatic
• Berechnetes Molekulargewicht: 12 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC017897
• Gene symbol: CLPS
• Gene ID (NCBI): 1208
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pancreatic colipase is a 12-kDa polypeptide cofactor for pancreatic lipase (EC 3.1.1.3), an enzyme essential for the absorption of dietary long-chain triglyceride fatty acids. Colipase is thought to anchor lipase noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts.