Calsyntenin-1 Polyklonaler Antikörper

Calsyntenin-1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 12788-1-AP

Synonyme

ALC ALPHA, Alcadein alpha, alcalpha1, alcalpha2, C terminal fragment 1 alpha, calsyntenin 1, Calsyntenin-1, CLSTN1, CS1, CSTN1, KIAA0911, PIK3CD, SAlc alpha CTF1 alpha, XB31alpha

---

Galerie der Validierungsdaten

• 

  WB analysis of human brain using 12788-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12788-1-AP (Calsyntenin-1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12788-1-AP

  IP Result of anti-CLSTN1 (IP:12788-1-AP, 4ug; Detection:12788-1-AP 1:300) with mouse brain tissue lysate 5600ug.

• 

  IHC staining of mouse brain using 12788-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12788-1-AP (Calsyntenin-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12788-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12788-1-AP (Calsyntenin-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

12788-1-AP bindet in WB, IP, IHC, IF, ELISA Calsyntenin-1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Calsyntenin-1 fusion protein Ag3504
• Vollständiger Name: calsyntenin 1
• Berechnetes Molekulargewicht: 109 kDa
• Beobachtetes Molekulargewicht: 109-130 kDa
• GenBank-Zugangsnummer: BC033902
• Gene symbol: CLSTN1
• Gene ID (NCBI): 22883
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calsyntenins (calsyntenin-1, -2, -3) compose a family of neuronally expressed proteins that belong to the cadherin superfamily of cell adhesion molecules (PMID: 24966372; 12498782). Calsyntenin-1 (CLSTN1) is a proteolytically processed postsynaptic membrane protein with a cytoplasmic calcium-binding domain. By binding Ca2+, calsyntenin-1 may modulate Ca2+-mediated postsynaptic signals (PMID: 11161476). Calsyntenin-1 is a transmembrane cargo-docking protein for Kinesin-1-mediated vesicular transport (PMID: 16760430). It can mediate axonal transport of the amyloid precursor protein and regulate Aβ production (PMID: 22434822).

Publikationen

Species	Application	Title
mouse	IF	J Neurosci; The cleavage products of amyloid-beta precursor protein are sorted to distinct carrier vesicles that are independently transported within neurites.; Authors - Muresan Virgil V
human	WB	Front Oncol; Long Non-coding RNA AK025387 Promotes Cell Migration and Invasion of Gastric Cancer.; Authors - Yi-Yuan Sun
rat	WB,IHC	Cardiovasc Drugs Ther; Calsyntenin-1 Promotes Doxorubicin-induced Dilated Cardiomyopathy in Rats; Authors - Mingxiang Zhu
mouse	WB	Cell Rep; A program of successive gene expression in mouse one-cell embryos; Authors - Maki Asami
human	WB	Cell Death Discov; ESRP1-driven alternative splicing of CLSTN1 inhibits the metastasis of gastric cancer; Authors - Chengguo Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Boyan (Verified Customer) (12-09-2019)

This antibody gave a clean band around 100 kD, like that is shown in its datasheet. However, in literature, people showed that this protein runs as doublet bands at 130 - 150 kD due to its glycosylation. Therefore, this antibody is highly suspicious for recognizing another protein rather than Clstn1 itself.

• Applications: Western Blot,